Pentas longiflora Oliv. (Rubiaceae), a plant used in the treatment of Pityriasis Versicolor in Rwanda: Chemical composition and standardization of leaves and roots

In Rwanda, the roots of Pentas longiflora Oliv. (Rubiaceae) have been used for a long time to treat Pityriasis versicolor . However, many people reported use leaves instead roots. This research was conducted compare phytochemical composition and establish chromatographic methods standardization extracts P. longiflora. During this process, three new pentalongin glycosides (pentalonginoside A, pentalonginoside B, C) two known same type (harounoside clarinoside), as well rutin, luteolin-7-rutinoside were isolated from methanol extract leaves. addition, psychorubrin, previously ethylacetate extract, also identified in extract. The presence antifungal compound may explain traditional treatment Pytiriasis Furthermore, harounoside, selected markers HPLC fingerprints MeOH accuracy risk profile demonstrated reliability validated method. general, considerable variations concentration plant metabolites, including pentalongin, observed between samples different sites. content (expressed juglone) collected ranged 1.7 70.0 mg/100 g. highest (70.0 ± 17 g) registered cultivated Mukoni. important variation concentrations according sampling sites, shows that order guarantee equivalent efficacy, finished products with should be standardized based on their content. • New heterosides Standardization medicinal plants. Validation analytical Chemical